1.PURPOSE To prepare the Smears as per standard text literature & Manufacture’s guidelines.

2.STAFF RESPONSIBLE Resident doctor

3.PRINCIPLE NA

4.PERFORMANCE SPECIFICATION NA

5.SAMPLE NA

6.TYPE OF CONTAINER NA

7.EQUIPMENT AND REAGENT USED

EQUIPMENTS

• Standard disposable 18-22 Gauze, 90 mm disposable lumbar puncture needle with trocar is suitable
• 10 cc Disposable Syringes.
• Disposable slides.
• 0.2mm cover slip.
• Diamond pencil for labeling the slides.

REAGENTS

• Fixative-Acetone free methanol – Extra pure

Stains

• H & E. Stain
• Giemsa Stain
• PAP Stain

8.CALIBRATION PROCEDURE

• When new residents come, they observe the procedure of FNAC for 3 days and then they perform FNAC under the observation of a senior resident for 4 days.
• After 1 wk of training, they are allowed to perform FNAC independently.

10. PROCEDURE (STEPS) PATIENT PREPARATION

• Explain procedure to patient to get his/her written consent and cooperation.
• Procedure is usually carried out as per position given by radiologist to the patient.
• Disinfect the skin using the spirit swab.
• Pre aspiration sedation is rarely necessary and the only in deep aspiration in anxious or agitated patients.

PROCEDURE

• Experienced radiologist find out lesion where FNAC has to be done.
• With the help of USG probe, operator position the needle within the target tissue.
• Pull the plunger to apply the negative pressure.
• Move the needle back and forth inside the target, so good part of aspirate is often drawn into the hub of needle.
• Release the negative pressure while needle remains in target tissue and withdraw the needle.
• Needle is detached and air is drawn into syringe.
• Blow sample on microscopy slide and with the help of other slides,spread the material.

11. QUALITY CONTROL PROCEDURE NA

13. REFERENCE INTERVALS NA

14. TAT FOR TEST Half hour

15. LABORATORY INTERPRETATION NA

16. ALERT CRITICAL VALUE NA

17.SAFETY PRECAUTION Universal safety precaution (Biosafety manual).

18.VALIDATION OF RESULTS TO BE DONE BY By authorized signatories &Assistant Technical manager.

19.REVIEW, RECORDS & RECOMMENDATION As per ISO:15189 Guidelines Clause 4.13 & NABL 112

20.Reference Fine Needle Aspiration Cytology, 4th edition, Svante R. Orell