1.PURPOSE To prepare the slide from received body fluid, sputum and BAL fluid

2.STAFF RESPONSIBLE Senior technician.

3.PRINCIPLE Sedimentation

4.REAGENT Glacial acetic acid

5.PERFORMANCE SPECIFICATION NA

6.SAMPLE Body fluid, BAL, Urine,Bladder wash, CSF

7.TYPE OF CONTAINER Wide mouth sterile container

8.EQUIPMENT AND REAGENT USED

• Conventional centrifuge, cytotek centrifuge,
• slides, applicator, WBC diluting, specimen, chamber holer, filter paper

9.CALIBRATION PROCEDURE Calibration of centrifuge is done regularly.

10. PROCEDURE (STEPS) ==A. Conventional centrifuge==

1. Take the fluid into the test tube.
2. In case of Hemorrhagic fluid, add equal volume of glacial acetic acid to the fluid.
3. Centrifuge it at 2000 rpm for 10 min.
4. Remove the supernatant, mix the sediment with the help of vortex mixture, take one drop on slide, prepare the smears.

11. QUALITY CONTROL PROCEDURE NA

13. REFERENCE INTERVALS NA

14. TAT FOR TEST Immediately processed

15. LABORATORY INTERPRETATION NA

16. ALERT CRITICAL VALUE NA

17.SAFETY PRECAUTION Universal safety precaution (Biosafety manual).

18.VALIDATION OF RESULTS TO BE DONE BY By authorized signatories &Assistant Technical manager.

19.REVIEW, RECORDS & RECOMMENDATION As per ISO:15189 Guidelines Clause 4.13 & NABL 112

20.Reference

1. Theory & practice of Histological Techniques, 4th edition, 1996, John D. Bancroft

1. Koss diagnostic Cytology & its Histopathologic bases, 5th edition, volume 2