• Learn operation of Erba XL-640 and Erbachem.
• Select equipment
  • Use Erba XL-640 as peferred equipment.
  • If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow procedure for responding to Erba XL-640 failure
• Read following documents

Note: Following instruction is useful only if operator is familiar with Erbachem

Note:

1. Run all testing material as sample (as described below at Sample Examination)
2. Evaluate calibration and QC absorbances in spreadsheet. Compare with past results.
3. If results are acceptable, enter the factor in the erbachem and set decimal place to zero.

1. Check aspiration volume of 300 microliter in test parameter of Total Billirubin.
2. Run 1 Randox Human assy control-2(QC 5),1 Randox Human assy control-3(QC 8) in each and every batch.See Assigned values of calibrators and target values of QC here
3. When instrument asks “Aspirate water”, aspirate water.
4. When instument ask for reagent blank, press 'No' (To prevent automatic correction of factor)

BLANK­

1. Take 400 microliter reagent R1 in ependroff cup.
2. Add 50 microliter sample to it.
3. Incubate at room temperature for 10 minutes.
4. Add 100 microliter DIAZO A onlyafter 10 minutes.
5. When instument ask for sample blank, aspirate it.

TEST

1. Take 400 microliter reagent R1 in ependroff cup.
2. Add 50 microliter sample to it.
3. Incubate at room temperature for 10 minutes.
4. Add 100 microliter R2 (Diazo A+Diazo B) after 10 minutes.
5. Take reading at 546nm.
6. Evaluate QC results. If QC results show major deviation from past, rerun batch.
7. If result comes more than 25mg/dl then dilute the sample about 1:1 with DI water and rerun the the sample.
8. Enter Calibration in LIS
9. Enter QC results manually in LIS
10. Enter sample results manually in LIS
11. instructions for determining quantitative results when a result is not within the measurement interval: Dilution of sample with DI water is done in same way as done by Erba XL-640 automatically.
12. |Note:For setting the test sequence of TBIl and DBIL, it should always be kept in mind that these two parameters should always be kept before phosphorus and calcium in the “TEST SEQUENCE” format as it would minimize the inference of phosphate buffer from glucose reagent, which earlier had been interfering with phosphorus.|