PROCEDURE FOR MAKING PEPSIN FOR ISE WASH ( current method )

PROCEDURE FOR MAKING PEPSIN FOR ISE WASH ( old method )

Checking Pepsin activity

  1. Take fibrin clot ( from PT tube/ clotted sample) .
  2. Take 2 small fibrin clots in 2 different glass tubes.
  3. Add 0.5 ml di water in 1 glass tube. This is control.
  4. Add 0.5 ml pepsin in another glass tube . This is test sample.
  5. Gently mix for 5 minutes at room temperature.
  6. If clot dissolve in test glass tube and not in control sample then Pepsin is active.