preparation of cholinesterase reagent
dissolve R1 in 30 ml and R2 in 1 ml of DI water
mix R1 and R2 after 15 min after complete dissolving of R1 and R2
aliquot 1 ml of reagent in plain vaccutte after proper washing of vaccutte
keep frozen
use 1 vaccutte of reagent as and when required
preserve the residual reagent after use