Reagent Handling

  1. Following steps will be done by user with prior training with Erba XL-640.
  2. Perform reagent refilling once every morning and in the afternoon and whenever required.
  3. Make sure that No RUN is going on
  4. Switch off power at the right side of XL-640 (light in front of XL-640 become RED) to prevent accidental injury by probe.
  5. Open reagent cover
  6. Refill/replace reagents as mentioned below in table.
  7. Close reagent cover. Match cover-guides properly.
  8. PRESS reagent cover at four place for proper fitting of cover to prevent bending of probe.
  9. switch on power at the right side of XL-640 (light in front of XL-640 become GREEN)
  10. Click reagent tray and then click volume scan.
  11. The screen will show available quantity of reagent and number of tests available.
  12. Referred to following table and take appropriate action.
Reagent Position Bottle size Examination Reagent Reagent Management How to refill
1 50 mlGlucose R1 KIT(R1=44ml) it is a system pack,kit bottle to be kept as it is, no top-ups
2 20 ml TG Kit 50 ml R1 and lyophilysed powder R2. Dissolve powder in R2 bottle with R1. Keep it for 10 min to dissolve. Mix R1 and R2 properly Refill up to 15 ml in morning
3 50 ml TBIL R1 IN HOUSE (MAKE 2 LITRE),filled in 50 ml bottles(system pack) . keep new bottle when machine shows reagent empty message
420 mlCALR1IN HOUSE (MAKE 2000 ml)Refill up to 15 ml in morning and evening
5 50 ml DBIL R1 IN HOUSE (MAKE 2 LITRE).filled in 50 ml bottles(system pack) . keep new bottle when machine shows reagent empty message
10 20 ml CHO R1 KIT(R1=60 ML) Refill up to 15 ml in morning
41,42 50ml,20mlALT R1=44ml R2=22mlit is a system pack, R1&R2 to be kept separately in machineDo not top up R1 or R2, always put new bottles
11,1250ml,20 mlUREAR1=44ml R2=11mlit is a system pack, R1&R2 to be kept separately in machineDo not top up R1 or R2, always put new bottles
28 20 mlCK-MBReady to Use MixR1+R2KIT (R1=2.5 ML R2=tablet).(RECONSTITUTE 2 BOTTLES OF BY ADDING one tab(R2) to one bottle of R1).MIX IT PROPERLY WAIT FOR 15 MINS.THEN TOP UP REAGENT BOTTLE WITHOUT DISCARDING OLD REAGENT)Refill 2.5 ml when volume scan <1 ml
1750 mlALBR1IN HOUSE (2 LITRE)filled in 50 ml bottles(system pack)keep new bottle when machine shows reagent empty message
1820 mlAMYR1=2ml NA2ml R1 bottles. Fill 1 bottle when instrument gives reagent empty message
6,820ml,20 mlALP,R1 and R2 separateIN HOUSE.MAKE 1 LITRE R1,250ml R2.Fill R1 and R2 separately. Do not top up R2,always wash R2 bottle before filling new reagentdiscard reagent when 26th cycle o.d >0.4
2220 mlTPR1IN HOUSE (MAKE 2 LITRE),filled in 20 ml bottles(system pack)keep new bottle when machine shows reagent empty message
2420 mlMPRR1IN HOUSE (MAKE 1 LITRE)Refill up to 10 ml only when equipment gives message
2620 mlUAReady to Use, R1 50ml+R2powder KIT (R1=50+R2=powder)Add 10 ml of R1 in R2 powder and wait for 10 min for powder to dissolve. Aliquate in 8ml bottles and store at 2-8 degre cRefill up to 1 bottle in morning and in evening
2150 mlCRR2IN HOUSE (MAKE 2 LITRE)Discard old and add new 30 ml
2350 mlCRR1IN HOUSE (MAKE 2 LITRE)Discard old and add new 30 ml
3020 mlTBIL(diazo)R2IN HOUSE (MAKE 2 LITRE).20ml Diazo A filled in 20ml system pack bottles, add 600ul diazo b and keep in instrument.fill tbilr2(diazo pack every 8 hours preferably just before analysing morning,evening and night qc's
1350 mlPHOR1=40mlINHOUSE(1litre).Refill in morning and evening
3820 mlCKReady to Use MixR1+R2KIT (R1=2.5 ML R2=tablet).(RECONSTITUTE 1 BOTTLE OF BY ADDING one tab(R2) to one bottle of R1).MIX IT PROPERLY WAIT FOR 15 MINS.THEN TOP UP REAGENT BOTTLE WITHOUT DISCARDING OLD REAGENT ( reagent to be kept in refrigerator, to be kept in instrument only when test has to be done)Refill 2.5 ml when volume scan <1 ml
35,3650 ml 20mlIRONR1=40ml,R2=4mlInhouse reagent(make 1000ml R1, 100ml R2). filled in 40ml and 20ml system pack bottleskeep in instrument when test to be done, after analysis to be stored in refrigerator.keep new bottle when machine shows reagent empty message
49,5050 ml 20mlUIBCR1=40ml,R2=4mlInhouse reagent(make 1000ml R1, 100ml R2). filled in 40ml and 20ml system pack bottleskeep in instrument when test to be done, after analysis to be stored in refrigerator.keep new bottle when machine shows reagent empty message
4420 mlLDHR1IN HOUSE.MAKE 500ML R1 AND 50 ML R2. aliquoted fixed 9ml R1 in 15ml bottles and fixed 1ml R2 in eppendroffcupsPrepare working reagent by mixing 9ml R1 with 1ml R2(Frozen cups) if volume scan<1 ml
4820 mlLIPASER1IN HOUSE (MAKE 500 ML)Discard residual reagent and refill fresh 5 ml reagent in morning
5350 mlDI WATERR1NARefill up to 40 ml
5550 mlWash Sol.R1NARefill up to 40 ml
5150ml Wash Sol. R1NARefill up to 40 ml
ISE-2(sample tray)1.5ml eppendroff cupPepsin NAfilled in 1.5ml ependroff cups, inhouse( 200ml) kept in deep freezer, kept in instrument after melting
ISE Reagent packstandard A bagelectrolytes(Na,K)standard A In House standard A(1000ml)fill 300ml to 350 ml of reagent into reagent pack(std A) through dispencer whenever it is empty
ISE Reagent packstandard B bagelectrolytes(Na,K)standard B In House standard B(1000ml)fill 300ml to 350 ml of reagent into reagent pack(std B) through dispencer whenever it is empty

Precautions taken during Reagent handling

  1. Never try to replace any reagent during run
  2. Allow examinations aborted if any reagent goes empty during run
  3. Allow examinations aborted if any sample goes empty during run