Preparation of sample loading buffer

  1. Take 10 ml of glycerol
  2. Add 25 ml of 0.1M EDTA, pH 6.8
  3. Add 1.21gm Tris base
  4. Add 6ml of 10% HCl
  5. Make up to 90 ml apporx. with DI water.
  6. Check pH, Adjust to 6.8 if required with 10% HCL
  7. Make up to 100ml.
  8. Add 40 mg of BPB dye.