Table of Contents

iron_reagent_final.ods

IRON examination procedure

referencesIron Reference literature
coral kit literature of Iron,TIBC
Note: When a new lot is opened, verify kit literature with the uploaded version.
Upload newer version when required and make necessary changes in procedure and process
environmental and safety controlsMSDS of sodium acetate
MSDS of acetic acid
MSDS of thiosemicarbazide
MSDS of ascorbicacid
MSDS of ferrozine
MSDS of MgCO3
Preparation of Iron and TIBC reagent
Preparation of IRON & UIBC _Current use

Erba XL-640

Centrifugation
Preparing aliquot
Procedure for examination in Erba XL-640

NOTE: ERBA XL 640 shows interference with iron assay when run randomly. Generally first sample in a batch also shows positive interferance. To prevent interferance, run iron and TIBC assay in saperate batch atleast once a day. This also helps in using fresh R1 with vitamin C. Program blank in Erba-XL 640. Program two types of Iron: 1) IRON 2) TIRON.

  1. Iron batch
  2. TIRON as first sample
  3. Iron as subsequent samples
  4. The equipment will take 3 TIRON blan, followed by 3 IRON blank. This TIRON will wash probe before measuring IRON.

Additional SOP

iron_tibc
sample_preparation_for_tibc
tibc_reagent
tibc