Tightly press over part as shown in below image so that top cover is movable and can be open and close easily.

Fill the Deionized water only upto mark as shown in below image

Put sample bag (Red bag) in bucket as shown in below image .As there are two bucket for sample,use two in case of more samples are there.

Partially attach all handle of top cover

Now tightly close all handles in order of opposite pattern of top cover.

-To start instrument, ON main switch labelled as no-1

Then ON switch, labelled as no-2

Then ON switch , labelled as no-3

Then press & hold on START button to see that green light will be visible.

Gren light will be shown as in below image

Now see display , If water is low , than it will show message of Low water

In that case ,open the instrument and fill water sufficient and than restart instrument

If water is sufficient ,display will show Temperature in degree Celsius and pressure

Initially temperature is 30 degree Celsius

Temperature will increase gradually ,after it reaches 90 degree Celsius ,ON release valve of top cover as shown in below image

Temperature will go upto 120 degree Celsius and pressure 15.2.

It will take 20 min to complete. (Time period can be set manually )

Afte 10 min of completion of autoclave , OFF release valve

From release valve and automatic valve all steam will be released.

Do not touch top cover during instrument is on and till instrument reaches to normal temperature.

For routine maintenance to remove water open yellow valve ,put tray below that and collect water,after each autoclave remove water and clean

Daily empty water from autoclave

Weekly clean autoclave inside by diluted HCL 10%