How to calibrate on Erba XL-640

  1. Analyze one sample after making new reagent,before calibration in order to check reagent blank and any gross mistake.
  2. Current calibration frequency policy
  3. prepare Randox cal 2 and cal 3
  4. Make new profile of parameter you want to calibrate in erba XL-640..
  5. Changes to make in new profile as shown in screenshot by click on 'edit' button.
    1. Decimal place = 4
    2. Sample replicate = 4
    3. K-Factor=1
    4. Reagents = New bottle.step2step 3
  6. For example,if you want to calibrate new reagent for Glucose, In reagents select NGLC R1.
  7. Calculate volume of sample and reagent.
  8. Reagent bottle preparation and programming of it in Erba XL-640.
Prepare for calibration as below when lot changes or there is relevent NC. Refer Calibrator and QC
testing materialnumber of cups
Water blank, 1)3
Normal Range Calibrator3
Abnormal range Calibrator3
Normal range QC sera3
Abnormal Range QC sera3
Put all samples in Erba XL-640
After calibration completed in Erba XL-640
  1. After Doing calibration as per above scheme , Export data from from Erba XL-640.Note file No. of exported file.
  2. After calibration completed , Open 10.207.3.245 and open exported file according to that file number.
  3. Sort data in Excel sheet as shown in example.Example of data sort in excel sheet
  4. Enter calibration in database.
  5. Consider 4 digit when writing factor.For example,in Albumin factor=6.121, TP factor=15.21 and Glucose factor=250.2.

Note:All samples should be done at least 10 times.

1)
5 gm/dl Bovine Serum albumin as blank in Creatinine