HDL cholesterol examination procedure
Learn operation of
Erba XL-640
and
Erbachem
.
Select equipment
Use Erba XL-640 as peferred equipment.
If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow
procedure for responding to Erba XL-640 failure
Read following documents
References
HDL reference literature
environmental and safety controls
MSDS of dextran sulphate
MSDS of Mgcl2
MSDS of Hcl
hdl_cholesterol_precipitator_reagent_new.ods
Erba XL-640
WDI for HDL-cholesterol
Multiply Cholesterol factor by 1.1|
HDL dilution factor calculation
| to arrive at HCL-C factor.
Due to 200 ul sample + 20 ul ppt reagent dilution
If any sample has more than 90mg/dl perform test with half dilution
It has been observed that abnormal range HDL Qc sample have more than 90mg/dl result. so,dilute it before performing abnormal QC.
Recently,calibration factor is used.Factor is derived by using Randox calibrators.
Centrifugation
Sample preparation for HDL
Procedure for examination in Erba XL-640