How to prepare HbA ,HBS and HBF control

For HBF Control preparation

  1. Collect about 2-4 ml cord blood in plastic bottle containing 2 ml CPDA
    1. Alternatively add washed RBC of new born EDTA sample in 2 ml CPDA bottle till total volume become 4 ml approximately. Preserve at 2-8'C till required amount is collected.
    2. Use washed RBC to prevent mismatch agglutination in pooled RBC
  2. Wash with saline twice in 4 ml EDTA vacumm tube. Remove Saline part
  3. After washing,Add Preservative CPDA in washed RBCs in 1:1 ratio.
  4. Prepare aliquots of 50 ul.
  5. Label and store at 2-8'C.

For HBA+HbS Control preparation

  1. Add washed RBC of HbA or HbS or HbA+HBS EDTA sample in 2 ml CPDA bottle till total volume become 4 ml approximately.
  2. Use washed RBC to prevent mismatch agglutination in pooled RBC
  3. Wash with saline twice in 4 ml EDTA vacumm tube. Remove Saline part
  4. After washing,Add Preservative CPDA in washed RBCs in 1:1 ratio.
  5. Prepare aliquots of 50 ul.
  6. Label and store at 2-8'C.

For HBA+HbS+HbF Control preparation

  1. Mixed HBA+HbS and HbF control in equal amount from above.
  2. Prepare aliquots of 50 ul.
  3. Label and store at 2-8'C.