Hemolysate

  1. 90 ml TEB Buffer (Half diluted).
  2. 2 ml Triton x -100
  3. 20 mg KCN
  4. 8 ml 0.1 M EDTA
  5. Mix well, Preserve it in fridge.

Acrylamide Hemolysate

  1. Take 1 gm acrylamide
  2. Add 10 ml hemolysate.
  3. Allow acrylamide to dissolve
  4. add 10 mg APS
  5. add 10 ul TEMED
  6. close lid
  7. allow polymerization for 30 minutes