• Learn operation of Erba XL-640 and Erbachem.
• Select equipment
  • Use Erba XL-640 as peferred equipment.
  • If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow procedure for responding to Erba XL-640 failure
  • If LIS is malfunctioning, print opd_entry_sheet.ods
• Read following documents

Note: When a new lot is opened, verify kit literature with the uploaded version. Upload newer version when required and make necessary changes in procedure and process|

• Document and revise common laboratory informations related to the examinations.
• Depending on the selected equipment follow appropriate procedure described below.

Note: Following instruction is useful only if operator is familiar with Erbachem

^Parameter^Value^

paper on lot to lot variationpaper_on_lot_to_lot_variation.pdf

1. Print examination wise worklist for glucose
2. Perform sample Centrifugation
3. Take 1 ml reagent in 1.5 ml eppendorff cup in required numbers and bring to room temprature(30 min)
4. Take 10 microliter sample in each tube at 30 second interval. Change tip every time.
5. incubate at room temprature for 1 hour.
6. Select “Run test” → Glucose in Erbachem menu.
7. When instrument asks “Aspirate water”, aspirate reagent instead. (To correct for absorbance of reagent blank)
8. When instument ask for reagent blank, press 'No' (To prevent automatic correction of factor)
9. take reading at 505 nm with 30 second interval.
10. Between two sample wash cuvet with water.
11. Take care not to exceed 30 second between reading of two samples.
12. Enter results( Actually absorbances) in spreadsheet
13. Evaluate factors and QC results. If factor/QC results show major deviation from past, rerun batch.
14. If sample absorbance more than 2.0 then dilute the sample about 1:1 with DI water and rerun the sample.
15. Calculate results=(Absorbance)X(Average Factor) in spreadsheet.
16. Enter Calibration in LIS
17. Enter QC results manually in LIS
18. Enter sample results manually in LIS
19. instructions for determining quantitative results when a result is not within the measurement interval: Dilution of sample with DI water is done in same way as autodilution done by Erba XL-640