lyophilized D-DIMER calibrator bottle is reconstituted with 1 ml(1 gm qc water) DI water, incubated for 10 to 15 minutes with periodic shaking until solutes becomes completely dissolved.
200 Ul of the mixture(reconstituted calibrator) is added with 200 Ul of Di water.
from there again 200 Ul of solution is added to the next alliquote and 200 Ul water is added for 3 more subsequent times. (serial dilution)
For establishing linearity a total of 6 samples are required.
1 sample is the plain calibrator(stock d-dimer), and the other one is DI water.
the samples are analysed in the analyser and od is plotted in the graph.
from there the factors are extracted and feeded in the machine.
For making an (IN use)ready to use reagent, 2000 uL of R1 is added with 500 uL of R2, which was already kept in fridge 2* to 8* C.
special wash reagent (0.1 n NAOH) is also added for wash