lyophilized crp bottle is reconstituted with 1 ml DI water, incubated for 10 to 15 minutes with periodic shaking until solutes becomes completely dissolved.
100 Ul of the mixture is added with 100 Ul of Di water.
from there again 100 Ul of solution is added to the next alliquote and 100 Ul water is added for 3 more subsequent times. (serial dilution)
For establishing linearity a total of 6 samples are required.
1 sample is the plain calibrator(as CRP stock), and the other one is DI water(as blank).
the samples are analysed in the analyser and od is plotted in the graph.
from there the factors are extracted and feeded in the machine.
the R1 volume is change from 200 UL to 160 ul and r2 volume is changed from 50 to 40 ul.
special wash reagent (0.1 n NAOH) is also added for wash