Table of Contents

Creatinine examination procedure

referencescreatinine_reference_literature_1
creatinine_reference_literature_2
creatinine_reference_literature_3
creatinine_reference_literature_For R1_1
creatinine_reference_literature_For R1_2
creatinine_reference_literature_For R1 with SDS
Note: When a new lot is opened, verify kit literature with the uploaded version.
Upload newer version when required and make necessary changes in procedure and process
environmental and safety controlsMSDS of NAOH
MSDS of BRIJ35%
MSDS of picric acid
MSDS of SDS
Preparation of Creatinine reagent

Special instructions for patients

The examination is done on Erba XL-640. It is also done on Erbachem when Erba XL-640 is damaged and repair may take longer

Erba XL-640

Erbachem

Note: Following instruction is useful only if operator is familiar with Erbachem

ParameterValue
Sample volume(ul)50
Reagent volume(ul)500
Filter505 nm
Aspitation Volume(ul)300
Type of reactionRATE-A
Decimal place1
Linearity1-20mg/dl
Delay time 30 sec
Reading time 60 sec
Calibration, Result calculation, Quality control and sample examination in Erbachem
Prepare for calibration as below every monthly or there is relevent NC. Refer Calibrator and QC
testing materialReplicates
5% Albumin blank4
Normal Range Calibrator4
Abnormal range Calibrator4
Normal range QC sera4
Abnormal Range QC sera4
  1. Run all testing material as sample (as described below at Sample Examination)
  2. Evaluate calibration and QC absorbances in spreadsheet. Compare with past results.
  3. If results are acceptable, enter the factor in the erbachem and set decimal place to zero.

sample examination

  1. Check aspiration volume of 300 microliter in test parameter of Creatinine.
  2. Run Randox Human assy control-2(QC 5),1 Randox Human assy control-3(QC 8) in each and every batch. See Assigned values of target values of QC here
  3. Select “Run test” → CRE in Erbachem menu.
  4. When instrument asks “Aspirate water”, aspirate water.
  5. When instrument asks “Aspirate sample”.
  6. Mix Creatinine reagent R1 and R2 in equal quantity.
  7. Take 500 microliter of mix (R1+R2) reagent in ependroff cup
  8. add 50 microliter sample to it
  9. Take reading immediately at 505 nm.
  10. Total time taken for analysis is 90 seconds. (delay time 30 seconds + reading time 60 seconds)
  11. Evaluate QC results. If QC results show major deviation from past, rerun batch.
  12. If result is more than 20.0mg/dl then dilute the sample with DI water and rerun the sample.
  13. instructions for determining quantitative results when a result is not within the measurement interval: Dilution of sample with DI water is done in same way as done by Erba XL-640 automatically.
  1. Currently serum and urine are accepted as sample type.