Check aspiration volume of 300 microliter in test parameter of Creatinine.
Run Randox Human assy control-2(QC 5),1 Randox Human assy control-3(QC 8) in each and every batch. See Assigned values of target values of QC
here
Select “Run test” → CRE in Erbachem menu.
When instrument asks “Aspirate water”, aspirate water.
When instrument asks “Aspirate sample”.
Mix Creatinine reagent R1 and R2 in equal quantity.
Take 500 microliter of mix (R1+R2) reagent in ependroff cup
add 50 microliter sample to it
Take reading immediately at 505 nm.
Total time taken for analysis is 90 seconds. (delay time 30 seconds + reading time 60 seconds)
Evaluate QC results. If QC results show major deviation from past, rerun batch.
If result is more than 20.0mg/dl then dilute the sample with DI water and rerun the sample.
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instructions for determining quantitative results when a result is not within the measurement interval: Dilution of sample with DI water is done in same way as done by Erba XL-640 automatically.