Creatinine
| Procedure for Creatinine measurement | nchsls:c:biochemistry:document:examination_procedure:LDH | Electroinc-only document | Edition (1) 27-01-2014 |
| Electronic copy of the document is stored at NCHSLS server, NCHS, Surat |
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| Creatinine Reference literature 1 |
| Creatinine Reference literature 2 |
| Creatinine Reference literature 3 |
The examination in serum and plasma is used mainly for diagnosis and management of renal disorders. Urine creatinine is frequently used as surrogate indicator to urine output
Creatinine reacts with alkaline picrate to form red colored complex. After an incubation period, increase in absorbance between two time points is measured at 490-540 nm (psudo-first order kinetics).The incubation period and time points for measurement of absorbance are chosen to decrease interference from other compounds reacting with alkaline picrate with different kinetics.
Linearity = 0-20[mg/dl] any one interval |Creatinine linearity check|
Within Batch Precision = <7.5[% CV]
Between Batch Precision(IQC) = <10[%cv]
EQA Peer Precision = <10[%cv]
Measuring_Interval = 0-40[mg/dl]
Trueness_of_Measurement = >40[TS]
Analytical_Sensitivity = 0.0315[A/mg/dl]
XL-640 Filter 340 nm
Temperature 37 degree C
Method Rate-A
Step-1 200 microliter R1
Step-2 10 microliter Sample
Step-3 M1S=0 M1E=0 M2S=3 M2E=8
Step-4 Reagent Absorbance Max=0
Step-5 Reagent Absorbance Min=0
Step-6 Reaction Absorbance limit=0
Step-7 Technical Min=0
Step-8 Technical Max=1000
Step-9 Linearity limit%=0
Step-10 Delta Abs/min%=0
Step-11 Reaction direction=Increasing
Internal Qc 3 times a day at 8 AM , 3 PM , 10 PM.
External Qc once in a month
More than 400[mg/dl Hemoglobin]
More than 30[mg/dl Total Bilirubin]
More than 12[mg/dl ascorbate]
More than 700[mg/dl Triglyceride]