1. Centrifuge fluoride and plain vacutte for 10 minutes at 3000 rpm. How to operate centrifuge
   

Observations:

1. If sample is highly hemolyzed,lipemic or icteric ,add examination interference in that sample id and write “sample is hemolyzed”.
2. If sample is highly hemolyzed , add examination rejection in that sample id and write “Sample is highly hemolyzed send new sample.
3. If any fluid is highly viscous so that it can block the probe, add examination rejection in that sample id and write “sample is too viscous for any analysis to be done”

Precautions:

1. Be careful about exchange of samples.
2. Do not use same tip to separate the serum. Use new tip for every sample.