-Lugol's iodine and acetic acid is asked by O.G. Dept. -This SOP helps to prepare both the reagents. -DO not prepare and dispatch these reagents without written request from H.O.D. of O.G. and permission from H.O.D Biochemistry. -For lesser number of patients prepare lesser amount of reagents.

1. Dissolve 5 gm Iodine crystals and 10 gm Potassium Iodide in 85 ml of DI Water.
2. Pour the solution in brown glass bottle allow it to dissolve overnight without using magnetic stirrer as Iodine get vaporised during mixing.
3. Note: Don't get confused with Iodine Tincture.Lugol's Iodine contains no alcohol while Iodine Tincture contains alcohol.
4. Do not re-issue brown bottles, ask resident to bring the bottles.
5. Label the bottle LUGOL'S IODINE, 100ml,Mgf. Date

1. Take 100ml D.I. water with volumetric flask in plastic bottle.
2. Remove 5ml D.I. water to make it 95 ml.
3. Add 5ml of glacial acetic acid.
4. Label the bottle 5% ACETIC ACID, 100ml,Mgf. Date

rh_vili.pdf
rh_via_evidence.pdf
principle_of_via_test.pdf

1. In phenol bottle- Two parts are there.
2. Upper part is Water saturated Phenol- Its concentration is 10%.
3. Lower part is Phenol saturated Water.
4. To prepare 5% phenol- Take 3 ml water saturated phenol(Upper part of bottle) and add 3 ml DI water.