Procedure for review of results

NameUnique ID Edition/Date of Edition
Procedure for review of resultsnchsls:c:biochemistry:document:14(1) 03-01-2014
Preparing authorityApproving authorityReview period
All teaching staff of biochemistry departmentHOD Biochemistry1 year
Printed copy of this document is considered uncontrolled. It should be compared with controlled electronic copy before use

Users

Generally review of results are done by resident doctors and technicians

Procedure

Before verification

During verification

CriteriaRemarks
High or low abnormal resultsverify properly all abnormal results very well
High direct bilirubin in samples from neonatal wardCall the ward doctors and discuss the laboratory findings
Absurd potassium and low calciumRepeat sample from primary tube —> if still absurd then call doctor and look for transfer of sample from flouride or EDTA to plain vacutte —> If yes then add 'Examination rejection' and ask for repeat sample.
If Alb < = 0.1 or creatinine < = 0.1Look for Sample cup in Erba XL-640 —> If sample is inadequete, repeat sample after refilling —> If same result comes —> Give sample probe wash and repeat the sample
If Alb < = 0.1 1.Check the sample type,If Fluid, it is possible. 2. If sample is serum,see the serum part & Cell Part(Hematocrit) of Vacuttee.If cell part is less ,sample may be diluted.So confirm the Hb value of Patient.
If ALT < = 0 or Negative Then result is given as below detection limit.
If Calcium < = 0 or Negative Then ,result is given as Absurd value.[Note : 1.First check the color of vacuttee,It must be plain otherwise calcium is chelated. It has been noticed that during collection of sample, blood is transfered in plain vacuttee from EDTA or Fluride. Check the K+ level, If it is transfered from EDTA, it is abnormally high. 2.It Should not be given as below detection limit.]
If potassium is absurd It may be due to EDTA contamination. How to check EDTA contamination (1)Check Calcium level, if it is absurd, EDTA contamination is confirm. (2) Check plain sample for adequecy of clotting. sample will not be clotted properly if EDTA contamination is there.
If Creatinine < = 0 or Negative First check the result of Billirubin.If It is < 15 mg/dl,give result as below detection.If it is > 15 mg/dl ,give result as Not Done & add remark as Hyperbillirubinemia Interfere with creatinine estimation.
If Total & Direct billirubin have same value.It is possible & give Indirect billirubin = 0 Or Below detection.Both values are correct .
If Direct bilirubin value<0.1.consider it as 0.0 and give Total bilirubin=Indirect bilirubin.For example, DBIL=-0.3, TBIL=0.6 so IBIL=0.6.
If Urea is high & Creatinine value is within normal range.Repeat the test,if it is same , Report should be released. Consult the clinician regarding their impression for patient's condition.[Note : It is possible in early stage of renal Disease.]
If sample is highly lipemic and interfere in test result1.Find out the cause of turbidity weather it is due to lipid or any other cause.
First do the test of TG. if it is high, ask for fasting sample or if it is fasting sample only, do the ultracentrifugation, perform test of albumin, TP, HDL and other parameters which was asked.
If ultracentrifugation is not possible at that time, do the test and release report with remarks that lipemia interfere with test result. If test results are negative e.g creatinine, release report as Not Done and add remarks that lipemia interfere with test result
If TG is normal, dilute the sample 1 time & perform the test. Consult the clinician and look for the other causes of lipemia e.g radioconcentration dye
If Calcium < 8.6 or > 10.2 Give calculated corrected calcium by using equation 0.8(4-alb)+ obtained calcium. Instrument will add albumin and corrected calcium reflexly Note: We are measuring total calcium = bound calcium + ionised calcium. When albumin is decrease, bound calcium is also decrease and free form of calcium remain same
Creatinine < 0.1 or negativeCreatinine result is <0.1.pdf
If potassium result is absuredFirst check the sample, wether it is hemolysed or not. If it is hemolysed, release the report as sample is hemolysed, and send repeat sample. Repeat the potassium ,if Result is same then do calcium.If calcium is <6.5 mg/dl,EDTA contamination may be present.If calcium is normal ,Pottasium result may or may be not absurd.corelate clinically & repeate the sample.
If sample is hemolysedResult of potassium will be high, Billirubin is negatively interfered(high Hb concentration breaks the azobilirubin complex), Protein is positively interfered(RBC has five times higher protein level than serum)
If Sodium >160mmol/l or <120mmol/l, Potassium is >8.0 mmol/l or <1.5mmol/lRepeat the sample again. Make sure that all other tests are done before repeating the electrolyte as the sample volume is 70 microliter.
If serum Iron is <20 ug/dl Manually write result as “<20”. Because LoD of Iron in our laboratory is 20 ug/dl.
CSF protein result reportingcsf_protein_test_reporting_edit-2.odp
if tp result's = or less than albuminthan repeat both albumin and tp, verify properly for any abnormal or low range results
If betaHCG result>15000In this condition instrument will do autodilution with dilution factor 100.So we have to wait for second result during verification of result.Second result have to be syncronised before release
If UIBC LESS THEN <0 OR NEGATIVEIf UIBC is negative then add remark as UIBC below detection so TIBC can not be calculated and correlate clinically for any recent oral and parentral iron therapy

*Note: Review of QC results is generally not required after complation of examination because they are reviewed before examination batch is started; in the event QC results are unexceptable, examination is not done in the first place. See individual examination procedures for further details . Maintenance sheet for Auto verification print