^Histopathology IQC Plan                                                        ^                                                                                                                                                                                                                                                                                                         ^                                                                        ^                                                                                                                                                                                                             ^                   ^
|No. of product groups                                                          |No. of tests/ calibration parameters                                                                                                                                                                                                                                                                     |Does the IQC plan broadly cover all tests/calibrations & details thereof|Frequency of IQC checks                                                                                                                                                                                      |Compliance criteria|
|1.Biopsy (small, medium & large tissues; Slides and blocks for second opinion):|* Grossing\\ * Decalcification\\ * Processing\\ * Paraffin embedding\\ \\ - Microtomy\\ \\ - H&E staining\\ \\ * \\ * Light Microscopy\\ \\ with interpretation                                                                                                                                          |                                                                        |-Lot validation with every change of reagent Lot\\ \\ - Section cutting and staining of each person\\ \\ Every 15 days\\ \\ - Daily QC for H&E staining\\ \\ - Inter observer quality control once in a month|                   |
|2.Histochemistry:                                                              |* PAS( Periodic Acid Schiff) stain\\ * Reticulin (Gordon and Sweet’s) stain\\ * Perl’s Prussian blue stain for Iron\\ * ZN (Ziehl Neelsen) stain for AFB\\ * Masson Trichrome stain\\ * Grocott Methanamine Silver Stain\\ * Alcian Blue stain\\ * Toludine Blue\\ \\ - Giemsa (May\\ \\ Grunwald Giemsa)|                                                                        |- IQC with each special stain\\ -interobserver comparision of all special stains every 6 months\\ \\ -Lot validation with every change of reagent lot                                                                                                                          |                   |
|3.Tissue received in normal saline:                                            |* Grossing\\ * Processing & section cutting by cryostat H & E staining                                                                                                                                                                                                                                   |                                                                        |Slides of each case for frozen section is sent for review by another authorised signatory posted in other section                                                                                            |                   |