====MICROTOME====

  *Bring the blocks on ice plate during cutting. Do rough cutting.
  *Trim the blocks after putting the blocks on the ice for 15 mins.
  *First smaller tissues are cut after that bigger tissues are cut.
  *Set temperature at 60c in water bath.
  *Insert the appropriate sharp knife (disposable) into the microtome at 45 degree angle.
  *Before making ribbon of section tight all the screw of microtome.
  *TO make the section ribbon set the thickness of about 5-7 micrometer.
  *The ribbon is directly transferred to water bath heated up to 5-6 degree below the melting point of wax.
  *Remove the creases from the ribbon and take the ribbon containing single large section or two to three sections on albumenized slide leaving preferably ¾ inch at one end for label and ½ inch on another end.
  *If during removing creases any damage to the section occur then ribbon should be discarded.   
  *Give the same number on the slide as on the block.
  *Put the slides rack in to the incubator at 60 degree temperature for 2 hrs.
  *Give xyline bath for clearing for 40 mins.
  *Rehydrate the sections by putting the slides in to increasing alcohol concentration and lastly in water.
  *Now stain the sections according to standard guidelines of different types of staining procedures.