=====19.SOP for In House Calibration=====

1.	**PURPOSE OF EXAMINATION: ** To calibrate instruments used in histopathology section.

2.	**PRINCIPLE AND METHOD OF PROCEDURE USED FOR EXAMINATION: **  set of readings are compared with calibrated reading mean is calculated.


3.	**STAFF RESPONSIBLE: ** Lab Technician

4.	**PERFORMANCE CHARACTERISTICS: ** NA

5.	**TYPE OF SAMPLE: ** NA


6.	**PATIENT PREPARATION: ** NA

7.	**TYPE OF CONTAINER AND ADDITIVES: ** Clean labelled plastic container


8.	**REQUIRED EQUIPMENT AND REAGENTS: ** NA

9.	**ENVIRONMENTAL AND SAFETY CONTROLS: ** Universal safety precautions 


10.	**CALIBRATION PROCEDURE: **NA

11.	**PROCEDURE(STEPS): **


•	In house calibration of following instruments is done 6 monthly.

1 Weighing Balance

2	Digital Thermometer

3	Tissue Embedding Station


4	Digital Thermometer

5	Digital incubator


6	Digital oven

7	Water Bath


8	Tissue floating Bath


•	Temperature based instruments calibrated by calibrated digital thermometer 

•	4 readings are taken then difference is noted and their mean is worked out.


•	Weighing Balance: calibrated weight of 1gm, 10mg and 100mg are used.

•	3 reading of balance are noted and correction factor is calculated and then mean is calculated.


12.	**QUALITY CONTROL PROCEDURE: ** calibrated equipments are used for calibration.

13.	**INTERFERENCE: ** NA


14.	**PRINCIPLE OF PROCEDURE FOR CALCULATING RESULTS INCLUDING, WHERE RELEVENT, THE MEASUREMENT OF UNCERTAINITY OF MEASURED QUANTITY VALUES: ** NA

15.	**BIOLOGICAL REFERENCE INTERVALS OR CLINICAL DECISION VALUES: ** NA


16.	**REPORTABLE INTERVAL OF EXAMINATION RESULTS: ** NA

17.	 **INSTRUCTION FOR DETERMINING QUANTITATIVE RESULTS WHEN RESULTS IS NOT WITHIN THE MEASUREMENT INTERVAL: ** NA


18.	**ALERT/ CRITICAL VALUE, WHERE APPROPRIATE: ** NA

19.	**LABORATORY CLINICAL INTERPRETATION: ** NA


20.	**POTENTIAL SOURCE OF VARIATION: ** NA

21.	**REFERENCES: ** Histopathological laboratory techniques by Bancroft, 6th edition