====FLUID PROCESSING====
  -**PURPOSE:** To prepare the slide from received body fluid, sputum and BAL fluid
  -**STAFF RESPONSIBLE:** Senior technician.
  -**PRINCIPLE:** Sedimentation
  -**REAGENT:** Glacial acetic acid
  -**PERFORMANCE SPECIFICATION:** 2000rpm for 10 mins
    *Linearity: NA
    *Precision and accuracy: NA
    *Sensitivity/specificity: NA
  -**SAMPLE:** Body fluid, BAL, Urine,Bladder wash, CSF
  -**TYPE OF CONTAINER:** Wide mouth sterile container
  -**EQUIPMENT AND REAGENT USED:** Conventional centrifuge, cytotek centrifuge,slides, applicator, WBC diluting, specimen, chamber holer, filter paper.
  -**CALIBRATION PROCEDURE:** Calibration of centrifuge is done regularly.
  -**PROCEDURE (STEPS):**
    *Conventional centrifuge
    *Take the fluid into the test tube.
    *In case of Hemorrhagic fluid, add equal volume of glacial acetic acid to the fluid.
    *Centrifuge it at 2000 rpm for 10 min.
    *Remove the supernatant, mix the sediment with the help of vortex mixture, take one drop on slide, prepare the smears.
  -**QUALITY CONTROL PROCEDURE:** When new
  -**REFERENCE INTERVALS:** NA
  -**TAT FOR TEST:** Immediately processed
  -**LABORATORY INTERPRETATION:** NA
  -**ALERT CRITICAL VALUE:** NA
  -**SAFETY PRECAUTION:** Universal safety precaution (Biosafety manual).
  -**VALIDATION OF RESULTS TO BE DONE BY:** By authorized signatories &Assistant  Technical manager.
  -**REVIEW, RECORDS & RECOMMENDATION:** As per ISO:15189 Guidelines Clause 4.13 & NABL 112
  -**Reference:** 
    -Theory & practice of Histological Techniques, 4th edition, 1996, John D. Bancroft
    -Koss diagnostic Cytology & its Histopathologic bases, 5th edition, volume 2