====WDI of H.E. Stain==== After preparing the smear Immediately fix it in methanol for 10 minutes. Dry the smears. First apply Haematoxylin for 2 minutes. Wash the slide 2 times in tap water. Apply Eosin for 5-10 seconds. Wash with tap water for 2 times. Dry the slides and mount with D.P.X. Label it properly