====SOP of Fluid processing====

**1.PURPOSE**
To prepare the slide from received body fluid, sputum and BAL fluid

**2.STAFF RESPONSIBLE**
 Senior technician.

**3.PRINCIPLE**
 Sedimentation


**4.REAGENT**
 Glacial acetic acid

**5.PERFORMANCE SPECIFICATION**
NA

**6.SAMPLE**
 Body fluid, BAL, Urine,Bladder wash, CSF

**7.TYPE OF CONTAINER**
 Wide mouth sterile container


**8.EQUIPMENT AND REAGENT USED**
  *Conventional centrifuge, cytotek centrifuge,
  *slides, applicator, WBC diluting, specimen, chamber holer, filter paper


**9.CALIBRATION PROCEDURE**
 Calibration of centrifuge is done regularly.

**10. PROCEDURE (STEPS)**
__==A. Conventional centrifuge==__
  -Take the fluid into the test tube.
  -In case of Hemorrhagic fluid, add equal volume of glacial acetic acid to the fluid.
  -Centrifuge it at 2000 rpm for 10 min.
  -Remove the supernatant, mix the sediment with the help of vortex mixture, take one drop on slide, prepare the smears.


**11. QUALITY CONTROL PROCEDURE**
NA

**13. REFERENCE INTERVALS**
 NA

**14. TAT FOR TEST**
 Immediately processed

**15. LABORATORY INTERPRETATION**
 NA

**16. ALERT CRITICAL VALUE**
 NA

**17.SAFETY PRECAUTION**
 Universal safety precaution (Biosafety manual).

**18.VALIDATION OF RESULTS TO BE DONE BY**
 By authorized signatories &Assistant  Technical manager.

**19.REVIEW, RECORDS & RECOMMENDATION**
 As per ISO:15189 Guidelines Clause 4.13 & NABL 112

**20.Reference**
  - Theory & practice of Histological Techniques, 4th edition, 1996, John D. Bancroft

  - Koss diagnostic Cytology & its Histopathologic bases, 5th edition, volume 2