====UNIVERSAL PRECAUTION==== 1.Protection of staff against laboratory infection *All laboratory personnel are informed of the hazards including transmission of viral infection involved in working in a laboratory. Possible route of infection may be skin abrasion or puncture or through body orifices. *In case of incidental exposure to infected (HIV reactive) samples like needle stick injury, wash the hand with soap &water and shall immediately be reported to authority. Use post exposure prophylaxis as per guidelines of regulatory authority. *Immunization of the laboratory staff against hepatitis-B infection is implemented. 2.Personnel Protection *Access to the lab is restricted to authorized personnel only. *All work surfaces are decontaminated after any spillage and at the end of each working day, *The staff is made aware of universal precautions as under **Hands ** -Wash hands before leaving the laboratory, and before eating or drinking. -Wash hands immediately after they have been in contact with blood or blood products. -Wear disposable gloves when handling all materials suspected of being infective or when opening samples of blood. -Keep open wounds and cuts covered with an adhesive dressing or disposable gloves during working. -Never invert a tube containing blood or a blood derivative by covering the mouth of the tube with a finger. - **Coats ** -Always use a clean lab coat with buttons closed- while working. -If a laboratory coat becomes -soiled with blood or serum, change to a clean one. The soiled coat should be soaked in hypochlorite or autoclaved before it is laundered. -Do not wear lab coat when you proceed for lunch. **Mouth ** -Do not put fingers or other objects such as pens, in the mouth. -Do not use mouth pipeting -Eating, drinking, smoking is not permitted in any working area of the laboratory. - No food or beverage be stored in any laboratory refrigerator, freezer, hot air oven or incubator. - Avoiding needle stick injuries. Never recap or bend needles Dispose all sharps in puncture proof containers.