====B12 Reagent Explanation====
  *  One uncoated cup from Reagent 2 is taken
  *  Serum added in it
  *  Alkanine reagent Reagent 3-1 is added
  *  Serum B12-Tc complex dissociate at alkaline pH and Tc get denatured (Reagent 3-1)
  *  Reagent 3-2 (neutralizing reagent) added
  *  On adding acid, pH is neutralized, but Tc remain denatured (Reagent 3-2)
  *  Stabilizing reagent from Reagent-2 added to buffer at specific pH?????
  *  Biotin-IF from Reagent 1 is added to uncoated cup
  *  Sample B12 bind Biotin-IF. Some Biotin-IF remain unbound
  *  [Sample B12-IF-Biotin complex]+[unbound Biotin-IF] from uncoated cup is taken and added to streptavidin-coated cup of Reagent-1
  *  SampleB12-IF-Biotin complex bind well
  *  B12-Free IF-Biotin bind well
  *  B12-HRP comjugated added.
  *  B12-HRP bind B12-Free IF-Biotin.
  *  Excess B12-HRP washed
  *  Reaction rate inversely proportion of B12 in serum

 -{{ :nchsls:c:biochemistry:document:b12_gem1350_ww_en_i_12.pdf |}} [kit literature of  Vit-B12 ]


======VITROS 3600======
|[[Centrifugation,  Preparing aliquot,  how_to_perform_examination_in_VITROS 3600]]| 

|[[INTERNAL QUALITY CONTROL]]| 

|[[EQA For VITROS_3600 Chemianalyzer Tests]]| 

{{ :nchsls:c:biochemistry:document:lot_verification_of_vitros-3600.ods |}}