======UIBC examination procedure======
  *Learn operation of [[Erba XL-640]] and [[Erbachem]].
  *Select equipment
    *Use Erba XL-640 as peferred equipment.
    *If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow [[procedure for responding to Erba XL-640 failure]]
  *Read following documents
  
^**reference**|{{ :nchsls:c:biochemistry:document:03702801.gif?nolink&600}}|
| |{{:nchsls:c:biochemistry:document:us4588695.pdf|}}|
| |{{:nchsls:c:biochemistry:document:good_iron_citratte_j._biol._chem.-2003-petrat-46403-13.pdf|}}|
| |{{:nchsls:c:biochemistry:document:nitilo_us5420008.pdf|}}|
| |{{:nchsls:c:biochemistry:document:nitrilo_156.full.pdf|}}|
| |{{:nchsls:c:biochemistry:document:d03123_.pdf|Thermoscintific Kit literature of UIBC}}|

| |**Note:** When a new lot is opened, verify kit literature with the uploaded version.|
| |[[lis_upload_dokuwiki_media|Upload]] newer version when required and make necessary changes in procedure and process|
{{ :nchsls:c:biochemistry:document:iron_uibc_reagent_2018.ods |}}

^**environmental and safety controls**|{{msds_sodiumacetate.pdf|MSDS of sodium acetate}}|
| |{{AceticAcidGlacial.pdf|MSDS of acetic acid}}| 
| |{{msds_thiosemicarbazide.pdf|MSDS of thiosemicarbazide}}|     
| |{{msds_ascorbicacid.pdf|MSDS of ascorbicacid}}|  
| |{{msds_of_ferrozine.pdf|MSDS of ferrozine}}| 
| |{{msds_MgCO3.pdf|MSDS of MgCO3}}|   
|Preparation of reagent{{:nchsls:c:biochemistry:document:iron_uibc_reagent_2018.ods|{{:nchsls:c:biochemistry:document:iron_uibc_reagent_2018.ods|}}}}


**Preparation of Aliquots**
  - Write sample id on ependroff cups with permanent marker pen on the rough surface of ependroff cup.
  - Arrange them in ascending order in the rack.
  - Aspirate serum from the centrifuged vacutte with the help of 200 uL pipette.
  - Dispense the serum into the respective sample id ependroff cup.
  - Recap the ependroff cup as soon as possible after dispensing serum.
  - Put ependroff cups with cap in refrigerator(2-8 ') if analysis is delayed.  
**Batch calibration in Erba-XL 640**
  - Put 1 cup of Blank
  - Put 1 cup of Cal 2
  - Put 1 cup of Cal 3
  - Put 1 cup of QC 5
  - Put 1 cup of QC 8
  - Run above Cups as sample for UIBC test and take 2 OD of each.
  - Export data in XL sheet and calculate factor.
**To Run Patient sample select NUIBC test**
  - You will get 26 th cycle OD.
  - Also note down 25th cycle OD in XL sheet so you get idea of interference of RBC in sample.If 25 th cycle od is high (> 0.4), see the sample. In hemolysed sample, 25th cycle OD will be high so do not release report and write comment in Remarks as " sample is hemolysed, send repeat sample.If sample is not hemolysed, centrifuge it again and repeat test. If sample is QNS, do not send result.ask for repeat sample. 
**Care to be taken while preparing ependrof cups for testing UIBC and IRON in ErbaXL**
  - centrifuge vacuttee, seprate serum carefully in ependrof cup and take care that RBCs are not to be present in seprated serum.
  - if sample seems turbid, centrifuge seprated serum further and then seprate serum from this cup in fresh another cup.
  - if there is huge lump in graph and later goes down after R2 it may probably show contamination with RBC.