======Procedure for TSH examination======
^references|{{ :nchsls:c:biochemistry:document:tsh_gem1001_ww_en_i_10_1.pdf |}}|

^**environmental and safety controls**|{{tsh_kit_literature.pdf|MSDS of TSH kit }}|

|[[Centrifugation]]|
|[[Sample preservation for TSH]]|

**How to perform [[TSH examination]]**

|[[Schedule for performing TSH and Free T4 examination]]|  

====== ** Instruction for TSH calibrator ** ======
   - 3 levels of calibrator ,in ready to use form ,liquid kept at 2 to 8 degree celcius temperature
   - for each level 7 allicorte of 300 microliter was made and parafilmed ,barcode label sticed and          kept in urine micro container and that  were kept in  box and labelled as **fresh calibrator cup**and put in deep fridge no 16
   - Left over sample, which was used for calibration , was kept in  original bottle of calibrator


======VITROS 3600======
|[[Centrifugation,  Preparing aliquot,  how_to_perform_examination_in_VITROS 3600]]| 

|[[INTERNAL QUALITY CONTROL]]| 

|[[EQA For VITROS_3600 Chemianalyzer Tests]]| 

{{ :nchsls:c:biochemistry:document:lot_verification_of_vitros-3600.ods |}}


=====Instruction for TSH reflex testing in instrument=====
  - For Thyroid profile, do entry of TSH only
  - Do not give Free T3 and Free T4 without permission of resident doctor
  - In instrument, do following setting to OFF reflex testing of Free T3 and Free T4
  - Go to Option 
  - Configure assay
  - Select TSH
  - Go to review and edit configuration
  - Following scrren will appaear
  - {{:nchsls:c:biochemistry:document:index-1.jpeg|}}
  - OFF reflex dilution
  - Click on configure reflex
  -  Following screen will appear
  - {{:nchsls:c:biochemistry:document:index-2.jpeg|}}
  - Deselect all test and deselect reflex to the same assay and reflexes to selected assay