10% TCA(TRI CHIORO ACETIC ACID) * Take 10 gm of 99% TCA in microcup approx.and immediately cap the bottle as it is in crystal form and very hygroscopic and weigh it on shimadzu machine for 10 gm. * Take approximately 50 ml of DI water in volumetric flask and add 10 gm of 99% TCA powder. * At the top of the flask paraffin it and mix throughly by shaking it updown several times. * Dissolve it properly and top-it-up upto 100 ml. * Do label the bottle appropriately, with concentration, quantity, manufacture date and expiry date. (NOTE:-Handle 99% TCA carefully as it is hygroscopic and immediately close the lid for reuse. Wear gloves and goggles as it can erode the skin)