====Preparation of CHE epindroff cups====

  - Put electronic pipette in charging 1 hour before starting procedure.
  - CHE kit provide R1 and R2.
  - Dissolve 30 ml DI water in R1 bottle and 1 ml DI water in R2 bottle.
  - Kept it for 15 min. Meanwhile keep ependroff cup label with 'CHE' and parafilm of appropriate size ready.
  - Mix 1 ml R2 in 30 R1 in bottle of R1. Total mix reagent volume is 31 ml.
  - Mix it well by gently invert and evert movement.
  - Fill 775 microliter of mixture (R1+R2) in ependroff cup with help of electronic pipette.
  - Make such approximately 40 cups and cover it with parafilm.
  - Store in deep freezer.
{{Biohit single channel electronic pipette.pdf|Biohit single channel electronic pipette user manual}}