**Preparation of Aliquots**
  - Write sample id on ependroff cups with permanent marker pen on the rough surface of ependroff cup.
  - Arrange them in ascending order in the rack.
  - Aspirate serum from the centrifuged vacutte with the help of 200 uL pipette.
  - Dispense the serum into the respective sample id ependroff cup.
  - Recap the ependroff cup as soon as possible after dispensing serum.
  - Put ependroff cups with cap in refrigerator(2-8 ') if analysis is delayed.