{{{{ :nchsls:c:biochemistry:document:pepsin_for_ise_wash.ods |}}|PROCEDURE FOR MAKING PEPSIN FOR ISE WASH ( current method )}}

{{:nchsls:c:biochemistry:document:old_method_for_preparation_of_pepsin_for_ise_wash.odt|PROCEDURE FOR MAKING PEPSIN FOR ISE WASH ( old method )}}


====preparation of pepsin rack====
{{ :nchsls:c:biochemistry:document:pepsin_rack.jpg |}}


====picture of how to filter reagent====
{{ :nchsls:c:biochemistry:document:filter_.jpg |}}




====Checking Pepsin activity====

  - Take fibrin clot ( from PT tube/ clotted sample) .
  - Take 2 small fibrin clots in 2 different glass tubes.which is shown below .clot in pepsin turn into dark red colour due to formation of acid hematin (reaction between hemoglobin with HCL present in pepsin)
  - {{ :nchsls:c:biochemistry:document:img_20200706_171634_-1_.jpg |}}
  - Add 0.5 ml di water in 1 glass tube. This is control.
  - Add 0.5 ml pepsin in another glass tube . This is test sample.
  - Gently mix for 5 minutes at room temperature. 
  - If clot dissolve in test glass tube and not in control sample then Pepsin is active. Dissolve clot decrease in size which is shown below.  
  - {{ :nchsls:c:biochemistry:document:img-2.jpg |}}