======Reagent Handling======
  - Following steps will be done by user with prior training with [[Erba XL-640]].
  - Perform reagent refilling once every morning and in the afternoon and whenever required.
  - Make sure that No RUN is going on
  - Switch off power at the right side of XL-640 (light in front of XL-640 become  RED) to prevent accidental injury by probe.
  - Open reagent cover  
  - Refill/replace reagents as mentioned below in table.
  - Close reagent cover. Match cover-guides properly.
  - PRESS reagent cover at four place for proper fitting of cover to prevent bending of probe.
  - switch on power at the right side of XL-640 (light in front of XL-640 become  GREEN)
  - Click reagent tray and then click volume scan. 
  - The screen will show available quantity of reagent and number of tests available.	
  - Referred to following table and take appropriate action. 

^Reagent Position ^Bottle size ^Examination ^Reagent ^Reagent Management ^How to refill^
|1 |50 ml|Glucose |R1 |KIT(R1=44ml) |it is a system pack,kit bottle to be kept as it is, no top-ups|
|2 |20 ml |TG |Kit 50 ml R1 and lyophilysed powder R2. |Dissolve powder in R2 bottle with R1. Keep it for 10 min to dissolve. Mix R1 and R2 properly |Refill up to 15 ml in morning|
|3 |50 ml |TBIL |R1 |IN HOUSE (MAKE 2 LITRE),filled in 50 ml bottles(system pack) . |keep new bottle when machine shows reagent empty message |
|4|20 ml|CAL|R1|IN HOUSE (MAKE 2000 ml)|Refill up to 15 ml in morning and evening|
|5 |50 ml |DBIL |R1 |IN HOUSE (MAKE 2 LITRE).filled in 50 ml bottles(system pack) . |keep new bottle when machine shows reagent empty message |
|10 |20 ml |CHO |R1 |KIT(R1=60 ML) |Refill up to 15 ml in morning |
|41,42 |50ml,20ml|ALT |R1=44ml R2=22ml|it is a system pack, R1&R2 to be kept separately in machine|Do not top up R1 or R2, always put new bottles |
|11,12|50ml,20 ml|UREA|R1=44ml R2=11ml|it is a system pack, R1&R2 to be kept separately in machine|Do not top up R1 or R2, always put new bottles |
|28 |20 ml|CK-MB|Ready to Use MixR1+R2|KIT (R1=2.5 ML R2=tablet).(RECONSTITUTE 2 BOTTLES OF BY ADDING one tab(R2) to one bottle of R1).MIX IT PROPERLY WAIT FOR 15 MINS.THEN TOP UP REAGENT BOTTLE WITHOUT DISCARDING OLD REAGENT)|Refill 2.5 ml when volume scan <1 ml|
|17|50 ml|ALB|R1|IN HOUSE (2 LITRE)filled in 50 ml bottles(system pack)|keep new bottle when machine shows reagent empty message |
|18|20 ml|AMY|R1=2ml| NA|2ml R1 bottles. Fill 1 bottle when instrument gives reagent empty message | 
|6,8|20ml,20 ml|ALP|,R1 and R2 separate|IN HOUSE.MAKE 1 LITRE R1,250ml R2.|Fill R1 and R2 separately. Do not top up R2,always wash R2 bottle before filling new reagent|discard reagent when 26th cycle o.d >0.4| 
|22|20 ml|TP|R1|IN HOUSE (MAKE 2 LITRE),filled in 20 ml bottles(system pack)|keep new bottle when machine shows reagent empty message|
|24|20 ml|MPR|R1|IN HOUSE (MAKE 1 LITRE)|Refill up to 10 ml only when equipment gives message|
|26|20 ml|UA|Ready to Use, R1 50ml+R2powder |KIT (R1=50+R2=powder)Add 10 ml of R1 in R2 powder and wait for 10 min for powder to dissolve. Aliquate in 8ml bottles and store at 2-8 degre c|Refill up to 1 bottle  in morning and in evening|
|21|50 ml|CR|R2|IN HOUSE (MAKE 2 LITRE)|Discard old and add new 30 ml|
|23|50 ml|CR|R1|IN HOUSE (MAKE 2 LITRE)|Discard old and add new 30 ml|
|30|20 ml|TBIL(diazo)|R2|IN HOUSE (MAKE 2 LITRE).20ml Diazo A filled in 20ml system pack bottles, add 600ul diazo b and keep in instrument.|fill tbilr2(diazo pack every 8 hours preferably just before analysing morning,evening  and night qc's |
|13|50 ml|PHO|R1=40ml|INHOUSE(1litre).|Refill in morning and evening |
|38|20 ml|CK|Ready to Use MixR1+R2|KIT (R1=2.5 ML R2=tablet).(RECONSTITUTE 1 BOTTLE OF BY ADDING one tab(R2) to one bottle of R1).MIX IT PROPERLY WAIT FOR 15 MINS.THEN TOP UP REAGENT BOTTLE WITHOUT DISCARDING OLD REAGENT ( reagent to be kept in refrigerator, to be kept in instrument only when test has to be done)|Refill 2.5 ml when volume scan <1 ml|
|35,36|50 ml 20ml|IRON|R1=40ml,R2=4ml|Inhouse reagent(make 1000ml R1, 100ml R2). filled in 40ml and 20ml system pack bottles|keep in instrument when test to be done, after analysis to be stored in refrigerator.keep new bottle when machine shows reagent empty message|
|49,50|50 ml 20ml|UIBC|R1=40ml,R2=4ml|Inhouse reagent(make 1000ml R1, 100ml R2). filled in 40ml and 20ml system pack bottles|keep in instrument when test to be done, after analysis to be stored in refrigerator.keep new bottle when machine shows reagent empty message|
|44|20 ml|LDH|R1|IN HOUSE.MAKE 500ML R1 AND  50 ML R2. aliquoted fixed 9ml R1 in 15ml bottles and fixed 1ml R2 in eppendroffcups|Prepare working reagent by mixing 9ml R1 with 1ml R2(Frozen cups) if volume scan<1 ml|
|48|20 ml|LIPASE|R1|IN HOUSE (MAKE 500 ML)|Discard residual reagent and refill fresh 5 ml reagent in morning|
|53|50 ml|DI WATER|R1|NA|Refill up to 40 ml|
|55|50 ml|Wash  Sol.|R1|NA|Refill up to 40 ml| 
|51|50ml |Wash Sol. |R1|NA|Refill up to 40 ml|
|ISE-2(sample tray)|1.5ml eppendroff cup|Pepsin |NA|filled in 1.5ml ependroff cups, inhouse( 200ml)| kept in deep freezer, kept in instrument after melting|
|ISE Reagent pack|standard A bag|electrolytes(Na,K)|standard A |In House standard A(1000ml)|fill 300ml to 350 ml of reagent into reagent pack(std A) through dispencer whenever it is empty|
|ISE Reagent pack|standard B bag|electrolytes(Na,K)|standard B |In House standard B(1000ml)|fill 300ml to 350 ml of reagent into reagent pack(std B) through dispencer whenever it is empty| 

======Precautions taken during Reagent handling======   
  - Never try to replace any reagent during run  	
  - Allow examinations aborted if any reagent goes empty during run	 
  - Allow examinations aborted if any sample goes empty during run