=== Preparation of sample loading buffer===
   -Take 10 ml of glycerol
   -Add 25 ml of 0.1M EDTA, pH 6.8
   -Add 1.21gm Tris base
   -Add 6ml of 10% HCl
   -Make up to 90 ml apporx. with DI water.
   -Check pH, Adjust to 6.8 if required with 10% HCL
   -Make up to 100ml.
   -Add 40 mg of BPB dye.