====Ketones====

===Reference===

==Preparation of Rothera's power==
  - Weight   following  chemical  &    mix    thoroughly  
  - Sodium carbonate ­­­­­­­= 20 gm 
  - Ammonium  sulfate  ­­­­= 20 gm
  - Sodium nitroprusside ­­­= 1 gm (make fine powder of it with mortar and pastle before mixing)
  - 
==Sample==
  -Urine
  -Serum(Blood) 

[[nchsls:c:biochemistry:document:online_information_resource_for_patients_and_users#Instructions_for_Patient_Collected_Samples|Special instructions for patients]]
==Procedure==
  -Take pinch of powder on a glass surface or a tile or a tile with well in it
  -Put a drop of sample on powder
  -Do not take sample more than what powder can absorb.
==Observation and interpretation==
  -If purple color observed, ketones are reported as "positive"
  -If no purple color observed, ketones are reported as "negative"
  -Read IQC (as shown below) to prevent confusion in detection of color

==Internal Quality Control==
  -Frequency of IQC : Whenever sample is received, Both the control (Positive & negative ) has to be run along with test.
  -Prepare 20 ml urine pool from OPD samples of apparently healthy individual. Divide in to two parts.
  -**Preparation of Positive control**
  -In 10 ml of pooled urine- add 10 ul acetone.
  -Prepare aliquotes  of 200 ul and give lot number e.g KP1, KP2, KP3....
  - -**Preparation of Negative control**
  - Take 10 ml of pooled urine
  - Prepare aliquotes  of 200 ul and give lot number e.g KN1, KN2, KN3....
  
  -Take powder in two wells.
  -In one well add negative control.
  -In another well add positive control.
  -Note observation in Ketones IQC Sheet
  -Upload sheet every month in LIS{{:nchsls:c:biochemistry:document:ketone_iqc_sheet.odt|Print sheet}}
  -The monthly QC sheet has to be uploaded in KTO calibration file.

==External Quality Control==
Currently there is  external quality Control done in the laboratory.