====Ketones====

===Reference===

==Preparation of Rothera's power==
  - Weight   following  chemical  &    mix    thoroughly  
  - Sodium carbonate ­­­­­­­= 20 gm 
  - Ammonium  sulfate  ­­­­= 20 gm
  - Sodium nitroprusside ­­­= 1 gm (make fine powder of it with mortar and pastle before mixing)
  - 
==Sample==
  -Urine
  -Serum(Blood) 

[[nchsls:c:biochemistry:document:online_information_resource_for_patients_and_users#Instructions_for_Patient_Collected_Samples|Special instructions for patients]]
==Procedure==
  -Take pinch of powder on a glass surface or a tile or a tile with well in it
  -Put a drop of sample on powder
  -Do not take sample more than what powder can absorb.
==Observation and interpretation==
  -If purple color observed, ketones are reported as "positive"
  -If no purple color observed, ketones are reported as "negative"
  -Read IQC (as shown below) to prevent confusion in detection of color

==Internal Quality Control==
  -Frequency of IQC : Whenever sample is received, Both the control (positive and Negative) has to be run along with test.
===Preparation of positive control===
  -Prepare pooled serum of 10 ml from OPD sample
  -Add 10ul Acetone in 10 ml of pooled serum
  -Give lot number as KP001, KP002,KP003...etc
  -Fill 50ul in each ependroff cup and seal it with parafilm and labeled as KP001, KP002,KP003...etc 
  -Store ependroff cups in refrigerator at 2-8 ' C.
===Preparation of Negative control===
  -Prepare pooled serum of 10 ml from OPD sample
  -Give lot number as KN001, KN002,KN003...etc
  -Fill 50ul in each ependroff cup and seal it with parafilm and labeled as KN001, KN002,KN003...etc
  -Store ependroff cups in refrigerator at 2-8 ' C. 
===How to do Quality control===
  -Take powder in two wells.
  -In one well add Positive control
  -In another well add Negative control.
  -Note observation in Ketones IQC Sheet
  -Upload sheet every month in LIS(Docs_Qualitative IQC){{:nchsls:c:biochemistry:document:ketone_sheet.ods|}}
  
==External Quality Control==
QA Forum sample for Ketone bodies done every 6 monthly.