{{:nchsls:c:biochemistry:document:iron_reagent_final.ods|}}
======IRON examination procedure======
  *Learn operation of [[Erba XL-640]] and [[Erbachem]].
  *Select equipment
    *Use Erba XL-640 as peferred equipment.
    *If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow [[procedure for responding to Erba XL-640 failure]]
  *Read following documents
  
^references|{{Iron_Reference_literature.pdf|Iron Reference literature}}|
| |{{:nchsls:c:biochemistry:document:iron_tibc_kit.pdf|coral kit literature of Iron,TIBC}}|
| |**Note:** When a new lot is opened, verify kit literature with the uploaded version.|
| |[[lis_upload_dokuwiki_media|Upload]] newer version when required and make necessary changes in procedure and process|

^**environmental and safety controls**|{{msds_sodiumacetate.pdf|MSDS of sodium acetate}}|
| |{{AceticAcidGlacial.pdf|MSDS of acetic acid}}| 
| |{{msds_thiosemicarbazide.pdf|MSDS of thiosemicarbazide}}|     
| |{{msds_ascorbicacid.pdf|MSDS of ascorbicacid}}| 
| |{{msds_of_ferrozine.pdf|MSDS of ferrozine}}| 
| |{{msds_MgCO3.pdf|MSDS of MgCO3}}|   

|Preparation of {{:nchsls:c:biochemistry:document:iron_reagent_final.ods|Iron and TIBC reagent}}| 
|Preparation of {{:nchsls:c:biochemistry:document:iron_uibc_reagent_2018.ods|IRON & UIBC _Current use}}|

======Erba XL-640======
|[[Centrifugation]]|
|[[Preparing aliquot]]|
|[[nchsls:c:biochemistry:document:how_to_perform_examination_in_erba_xl-640|Procedure for examination in Erba XL-640]]|


**NOTE:** ERBA XL 640 shows interference with iron assay when run randomly. Generally first sample in a batch also shows positive interferance. To prevent interferance, run iron and TIBC assay in saperate batch atleast once a day. This also helps in using fresh R1 with vitamin C. Program [[blank in Erba-XL 640]]. Program two types of Iron: 1) IRON 2) TIRON.  
  -Iron batch
    -TIRON as first sample
    -Iron as subsequent samples
    -The equipment will take 3 TIRON blan, followed by 3 IRON blank. This TIRON will wash probe before measuring IRON.  

====Additional SOP====
[[iron_tibc]]\\
[[sample_preparation_for_tibc]]\\
[[tibc_reagent]]\\
[[tibc]]