{{:nchsls:c:biochemistry:document:how_to_use_riqas_website_instruction_.pdf}}

Details of EQC program===
  - Open "www.riqas.net" website from computer on which internet available.
  - Following window open.{{riqas_1.png|step 1}}
  - Click on login.Enter user name and password.User name = biochemisty password=???
  - Following window opens. {{:nchsls:c:biochemistry:document:riqas.png|}}
  - Go to "Data entry"---->"Enter results".Following window opens.{{:nchsls:c:biochemistry:document:riqas.png|}}
  - Click on "123049/A" and following window opens.{{riqas_4.png|step 4}}
  - In above window , we can know the last date of posting external Qc results.
  - Note down or print this page to prevent last day rush in posting EQC results.
===Preparation of EQC Samples===
  - Take bottle of EQC from refrigerator.Make sure to check sample number according to month.for example , If you want to prepare EQC sample for **june month then sample number=6** bottle should be taken.
  - Do not get confused between cycle Number and sample number.
  - It has been found that the laboratory made mistake in reconstituting correct sample number.To prevent this error in future ,take following steps.
    - Cross check correctness of sample with one more person.
    - Preserve empty bottle of sample till report arrives.
  - Take 5 ml Qc water(DI water of 0.00 microsiemens/cm) in volumetric flask.
  - Gently transfer water in EQC bottle.Pour last drop of water by simply rotate flask over edge of bottle.Do not beat the flask over bottle.
  - Mix it by gentle inversion and eversion.
  - Put it at room temperature for 30 minute and watch for all powder particle has been dissolved.
  - Fill ependroff cup as per your need and seal with it parafilm.
 
^Numbers of ependroff cup^Ependroff cup quantity^Test to perform^
|3|200 micriliter|For HDL cholesterol|
|3|200 micriliter|For TIBC|
|2|200 micriliter|For TSH|
|2|100 micriliter|For Free T4|
|1|600 micriliter|For Semiauto all parameters and prolyte|
|5|500 micriliter|For Erba XL-640 all parameters|
|1|leftover sample|For future use|

===Doing EQC===
  - Run sample of EQC in morning with first batch in Erba XL-640 for 4 days.
  - DO TSH and free T4 2 times with each ELISA batch of TSH and Free T4.
  - Do HDL cholesterol manually 3 times when you do patient sample of TIBC and HDL cholesterol.
  - Do Semiauto EQC single time after calibrating semiauto for Glucose,ALT,Creatinine and bilirubin.
  - Sort data in excel sheet and calculate average ,SD and CV.
  - 5th cup of 500 microliter is preserved for any problem in any parameter and we want to repeat it.

===Post results===
  - Open "www.riqas.net" website from computer on which internet available.
  - Following window open.{{riqas_1.png|step 1}}
  - Click on login.Enter user name and password.User name = biochemisty password=150003
  - Following window opens.{{riqas_2.png|step 2}}
  - Go to "Data entry"---->"Enter results".Following window opens.{{:nchsls:c:biochemistry:document:riqas.png|}}
  - Click on "129049/A" and following window opens.{{:nchsls:c:biochemistry:document:riqas2.png|}}
  - Click on "sample number" to post results.{{:nchsls:c:biochemistry:document:riqas44.png|}}
  - result upload on website after verification.
  - After upload of result on website, Re-verified by third person that not present during result upload.
  - click on "ok" button.{{:nchsls:c:biochemistry:document:riqas_ok.png|}}
  - 
  - Do not communicate with other participants in the interlaboratory comparison programme about sample data until after the date for submission of the data.
  - Do not refer interlaboratory comparison samples for confirmatory examinations before submission of the data, although this would routinely be done with patient samples. 

===Monitoring results===
  -[[Analyse EQA and IQC results]] every month.  
  -Whenever possible compare results with mean of method because very small number of laboratory uses Erba XL-640. 
  -Root cause analysis(RCA) of EQA done for that parameter which has SDI > 2 or < 40 target score.{{:nchsls:c:biochemistry:document:rca_protocol.ods|RCA Protocol sheet}}
  -Record of RCA is available in IQC analysis file of its respective month and it is also available in LIS--> Non conformance.