* We Have ready made, lyophilized, dry powder formed calibrator with two different concentration of D-DIMER. See photograph {{:nchsls:c:biochemistry:document:2ddimer.jpeg?400|}} {{:nchsls:c:biochemistry:document:3200.jpeg?400|}} {{:nchsls:c:biochemistry:document:5420.jpeg?400|}} - 3200 ng/ml - 5420 ng/ml * We are using Normal Saline (NS) for reconstitution purpose. * For reconstitute the Calibrator with 3200 ng/ml concentration, add 1 ML of NS into dry calibrator. So resulted concentration will be of 3200 ng/ml. * For reconstitute the Calibrator with 5420 ng/ml concentration, add 1700 uL of NS into dry calibrator. So resulted concentration will be of 3200 ng/ml. * This is our stock solution. * **__How to make QC:-__** * We decided to make QC of 800 ng/ml concentration from this stock solution. * For making such QC, take 800 uL of stock solution. * Add 2200 uL of Normal Saline. * Total volume will be of 3000 uL (3 ML). * From this 3 ml, Make cups of 200 uL. * 15 cups will be made. * Use these cups as QC.