====How to calibrate on Erba XL-640====
  - Analyze one sample after making new reagent,before calibration in order to check reagent blank and any gross mistake.
  - [[nchsls:c:biochemistry:document:calibration_and_quality_control_frequency|Current calibration frequency policy]] 
  - [[prepare Randox cal 2 and cal 3]]
  - [[Make new profile]] of parameter you want to calibrate in erba XL-640..
  - Changes to make in new profile as shown in screenshot by click on 'edit' button.
      - Decimal place = 4
      - Sample replicate = 4
      - K-Factor=1  
      - Reagents = New bottle.{{profile 1.JPG|step2}}{{profile 2.JPG|step 3}}
  - For example,if you want to calibrate new reagent for Glucose, In reagents select NGLC R1.
  - Calculate volume of sample and reagent.
  - [[Reagent bottle preparation and programming of it in Erba XL-640]].

^Prepare for calibration as below when lot changes or there is relevent NC. Refer [[Calibrator and QC]]^
^testing material^number of cups^
|Water blank, ((5 gm/dl Bovine Serum albumin as blank in Creatinine))|3|
|Normal Range Calibrator|3|
|Abnormal range Calibrator|3|
|Normal range QC sera|3|
|Abnormal Range QC sera|3|
^Put all samples in Erba XL-640^

==After calibration completed in Erba XL-640==
  - After Doing calibration as per above scheme , Export data from from Erba XL-640.Note file No. of exported file.
  - After calibration completed , Open 10.207.3.245 and open exported file according to that file number.
  - Sort data in Excel sheet as shown in example.{{2014-07-23_calibration_ALB_027_ERBAXL.ods|Example of data sort in excel sheet}}
  - [[Enter calibration in database]].
  - Consider 4 digit when writing factor.For example,in Albumin factor=6.121, TP factor=15.21 and Glucose factor=250.2.

**Note**:All samples should be done at least 10 times.