======HDL cholesterol examination procedure======
  *Learn operation of [[Erba XL-640]] and [[Erbachem]].
  *Select equipment
    *Use Erba XL-640 as peferred equipment.
    *If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow [[procedure for responding to Erba XL-640 failure]]
  *Read following documents

^References|{{DS-MG2+ HDL.pdf|HDL reference literature}}| 

^**environmental and safety controls**|{{msds_dextran sulphate.pdf|MSDS of dextran sulphate}}|
| |{{msds_Mgcl2.pdf|MSDS of Mgcl2}}|
| |{{msds of Hcl.pdf|MSDS of Hcl}}| 

{{ :nchsls:c:biochemistry:document:hdl_cholesterol_precipitator_reagent_new.ods |}}
======Erba XL-640======
  *{{:nchsls:c:biochemistry:document:wdi_for_hdl-cholesterol.odt|WDI for HDL-cholesterol}}
  *Multiply Cholesterol factor by 1.1|{{:nchsls:c:biochemistry:document:hdl_dilution_factor_calculation.ods|HDL dilution factor calculation}}| to arrive at HCL-C factor.
  *Due to 200 ul sample + 20 ul ppt reagent dilution  
  *If any sample has more than 90mg/dl perform test with half dilution
  *It has been observed that abnormal range HDL Qc sample have more than 90mg/dl result. so,dilute it before performing abnormal QC. 
  *Recently,calibration factor is used.Factor is derived by using Randox calibrators.

|[[Centrifugation]]|
|[[Sample preparation for HDL]]|
|[[nchsls:c:biochemistry:document:how_to_perform_examination_in_erba_xl-640|Procedure for examination in Erba XL-640]]|