-Take approx. 600 ml of DI water in 2 liter flask 
  -Add 10.2 gm Tris base
  -Add 3.2 gm boric acid
  -Add 0.2 gm EDTA powder
  -Mix properly with stirrer and do upto 2 liter
  - Measure the Ph of final buffer and it would be 8.6