===Hemolysate===
  -90 ml TEB Buffer (Half diluted). 
  - 2 ml Triton x -100  
  - 20 mg KCN 
  - 8 ml 0.1 M EDTA
  - Mix well, Preserve it in fridge.
===Acrylamide Hemolysate===
  -Take 1 gm acrylamide
  -Add 10 ml hemolysate.
  -Allow acrylamide to dissolve
  -add 10 mg APS
  -add 10 ul TEMED
  -close lid
  -allow polymerization for 30 minutes