======Glucose examination procedure======
  *Learn operation of [[Erba XL-640]] and [[Erbachem]].
  *Select equipment   
    *Use Erba XL-640 as peferred equipment.
    *If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow [[procedure for responding to Erba XL-640 failure]]
    *If LIS is malfunctioning, print {{ :nchsls:c:biochemistry:document:opd_entry_sheet.ods |}} 
  *Read following documents

|**References**|| 
|{{glucose_kit_literature_transasia.pdf|Glucose Kit literature of Transasia}}|
|{{Glucose Randox kit literture .pdf|Glucose Kit literature of Randox}}|
 **Note:** When a new lot is opened, verify kit literature with the uploaded version. [[lis_upload_dokuwiki_media|Upload]] newer version when required and make necessary changes in procedure and process|
|**environmental and safety controls**|
|{{msds_transasia_kits.pdf|MSDS of Transasia Kits}}|
|{{:nchsls:c:biochemistry:document:msds_of_randox_kits.pdf|MSDS of randox kits}}|

  *Document and revise [[common laboratory informations related to the examinations]].
  *Depending on the selected equipment follow appropriate procedure described below.
======Erba XL-640======
|[[Centrifugation]]| 
|[[Preparing aliquot]]|
|[[nchsls:c:biochemistry:document:how_to_perform_examination_in_erba_xl-640|Procedure for examination in Erba XL-640]]|
|[[ Intereference by GOD POD Glucose Reagent]]|  
======Erbachem======
**Note:** Following instruction is useful only if operator is familiar with [[Erbachem]]
  
^Parameter^Value^
^Mode|1-POINT|
^Filter|505 nm|
^Factor|1|
^Delay T|5|
^Read T|0|
^Temperature|37 'C|
^Unit|mg/dl|
^Sample volume(ul)|10|
^Reagent volume(ul)|1000| 
^Aspitation Volume(ul)|800, Very important|
^Decimal place|4| 


^Calibration, Result calculation, Quality control and sample examination in Erbachem^

^[[nchsls:c:biochemistry:document:internal_quality_control|Internal Quality Control]]^
^[[how_to_do_external_quality_control_on_erba_xl-640|EQA]]^
^{{:nchsls:c:biochemistry:document:lot_verification.ods|}}Lot Varification|^
paper on lot to lot variation{{ :nchsls:c:biochemistry:document:paper_on_lot_to_lot_variation.pdf |}}


  -[[Print examination wise worklist]] for glucose
  -Perform sample [[Centrifugation]]
  -Take 1 ml reagent in 1.5 ml eppendorff cup in required numbers and bring to room temprature(30 min) 
  -Take 10 microliter sample in each tube at 30 second interval. Change tip every time.			
  -incubate at room temprature for 1 hour.	 	
  -Select "Run test" -> Glucose in Erbachem menu.
  -When instrument asks "Aspirate water", aspirate reagent instead. (To correct for absorbance of reagent blank)
  -When instument ask for reagent blank, press 'No' (To prevent automatic correction of factor)
  -take reading  at 505 nm with 30 second interval.
  -Between two sample wash cuvet with water.
  -Take care not to exceed 30 second between reading of two samples.
  -Enter results( Actually absorbances) in spreadsheet
  -Evaluate factors and QC results. If factor/QC results show major deviation from past, rerun batch.
  -If sample absorbance more than 2.0 then dilute the sample about 1:1 with DI water and rerun the sample. 
  -Calculate results=(Absorbance)X(Average Factor) in spreadsheet.
  -[[lis_calibration_entry|Enter Calibration in LIS]]
  -[[list_qc_entry_manual|Enter QC results manually in LIS]]
  -[[list_result_entry_manual|Enter sample results manually in LIS]]
  -**instructions for determining quantitative results when a result is not within the measurement interval:** Dilution of sample with DI water is done in same way as {{lis_Test Volumes.ods|autodilution done by Erba XL-640}} 

  -{{ :nchsls:c:biochemistry:document:glucose_principle_manually_.png |}}

LOL