- The interior of the autoclave is filled with water till a defined level.
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  - An empty bucket is kept in the autoclave.
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  - The red bag containing vacuttes , tips, ependroff cups, etc. is kept in the bucket.
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  - Thereafter, autoclave is switched on after placing the lid.
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  - It takes around 30mins to attain a pressure of 1-1.5 kg/cm sq.
  - When the pressure is obtained, the safety whistle will be raised and a sound will be heard.
  - Switch off the autoclave
  - Lid of autoclave  should not open atleast  for 30mins.