======Electrolyte examination procedure====== ^references|{{erba_electrolyte_pack.pdf|Na+ Reference literature 1}}| ^**environmental and safety controls**|{{MSDS-sodium-chloride.pdf|MSDS of sodium chloride}}| | |{{msds_Potassium chloride.pdf|MSDS of Potassium chloride}}| | |{{msds_Lithium carbonate.pdf|MSDS of Lithium carbonate}}| **Preparation of [[Electrolyte reagent]]**\\ **Preparation and use of [[Electrolyte reagent]]** **[[Filling of Electrolyte pack]]** **[[Maintenance after filling fluid pack]]** -Absurd values for Na & K{{:nchsls:c:biochemistry:document:critical_laboratory_values_smhc.pdf|}} **How To analysed electrolyte in different sample types** - {{:nchsls:c:biochemistry:document:how_to_analysed_electrolyte_in_different_sample_types.ods|}} **Preparation of Aliquots** - Write sample id on ependroff cups with permanent marker pen on the rough surface of ependroff cup. - Arrange them in ascending order in the rack. - Aspirate serum from the centrifuged vacutte with the help of 200 uL pipette. - Dispense the serum into the respective sample id ependroff cup. - Recap the ependroff cup as soon as possible after dispensing serum. - Put ependroff cups with cap in refrigerator(2-8 ') if analysis is delayed. ===Measurement of urine sample=== -Equipment takes 225 ul MOPS dilutent in two cuvettes -Then 25 ul urine is added in both -From both cuvettes 70ul sample is taken in electrode channels. -First 70ul is used for washing -2nd 70ul is used for analysis