======Frequency of calibration and IQC======
^  Erba XL-1000  ^^^^^^
^Parameter^Calibration^IQC^Calibration Policy^Correlation Factor^EQC Policy^  
^  calibrator and Replicates/QC and Replicates  ^^^^^^
^  prepare 200ul calibratuo cups and devide 500ul QC cups in to 200ul  ^^^^^^
^  perform recalibration of same lot if qc results are out of 1SD on same side for more than 15days  ^^^^^^
|GLC|WHEN LOT CHANGEs or if calibration is older than 1 month even if lot is same|2 LEVEL IQC at 8 AM ,3 PM , 10 PM|3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax+b**|RIQAS_monthly| 
|Sodium|whenever electrodes changed.|Do 2 level IQC 5 times when fluid pack refilled and 2 level IQc at 8 AM ,3 PM , 10 PM|5 calibrator CUPS X 2 time replicate and 5 IQC cups x 2 time replicate|**y=ax**|RIQAS_monthly|
|Potassium|whenever electrodes changed.|Do 2 level Qc 5 times when fluid pack refilled and 2 level Qc at 8 AM ,3 PM , 10 PM|5 calibrator CUPS X 2 time replicate and 5 QC cups x 2 time replicate|**y=ax**|RIQAS_monthly|
|TBIL|WHEN LOT CHANGES|2 LEVEL IQC at 8 AM ,3 PM , 10 PM|**Use freshly prepared calibrators and quality** **control(after one hour of reconstitution)**3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax+b**|RIQAS_monthly|
|DBIL|WHEN LOT CHANGES|2 LEVEL IQC at 8 AM ,3 PM , 10 PM|**Use freshly prepared calibrators and**Use freshly prepared calibrators and3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate|**Use TBIL Factor achieved in calibration**|RIQAS_monthly|
|ALT|WHEN LOT CHANGES|2 LEVEL IQC at 8 AM ,3 PM , 10 PM|3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax+b**|RIQAS_monthly|
|TG|WHEN LOT CHANGES or if calibration is older than 1 month even if lot is same|2 LEVEL IQC at 8 AM ,3 PM , 10 PM|3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax+b**|RIQAS_monthly|
|CHO|WHEN LOT CHANGES or if calibration is older than 1 month even if lot is same|2 LEVEL IQC at 8 AM ,3 PM , 10 PM|3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax+b**|RIQAS_monthly|
|UREA|WHEN LOT CHANGES |2 LEVEL IQC at 8 AM ,3 PM , 10 PM|3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax+b**|RIQAS_monthly|
|UA|WHEN LOT CHANGES|2 LEVEL IQC at 8 AM ,3 PM , 10 PM|3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax+b**|RIQAS_monthly|
|CKMB|Fixed Factor,adjust factor according to dilution factor when kit/protocol changed based on molar absorptivity of NADPH|KIT CONTROL WHEN BOTTLE CHANGE.2 LEVEL IQC AT 8 AM, 1 LEVEL IQC AT 3 PM, 1 LEVEL IQC AT 10 PM| NA.|Fixed Factor| |
|ALB|WHEN LOT CHANGES|2 LEVEL IQC at 8 AM ,3 PM , 10 PM|3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicated|**y=ax+b**|RIQAS_monthly|
|ALP|WHEN LOT CHANGES|2 LEVEL IQC at 8 AM ,3 PM ,10 PM|3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax+b**|RIQAS_monthly|
|TP|WHEN LOT CHANGES|2 LEVEL IQC at 8 AM ,3 PM , 10 PM|3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax+b**|RIQAS_monthly|
|AMY|WHEN LOT CHANGES|2 LEVEL QC at 8 AM ,3 PM , 10 PM|3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax+b**|RIQAS_monthly|
|CAL|When Lot changes because reagent is used up within 35 days|2 LEVEL QC at 8 AM ,3 PM , 10 PM (In duplicate and second value is considered)|3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax+b**|RIQAS_monthly|
|CAL(OCPC)|Every four hourly|2 LEVEL QC 4 Hourly and with each new batch(In duplicate and second value is considered)|4 calibrators(HAlf cal 2, half cal3, cal 2, cal 3) cups X 2 time replicate, one blank x 2 times| **y=ax+b**|RIQAS_monthly|
|CR|WHEN LOT CHANGES|2 LEVEL QC at 8 AM ,3 PM ,10 PM|3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 albumin(5%) blank cups X 4 time replicate|**y=ax+b(Dont Use the value of blank for finding the equation)**|RIQAS_monthly|
|LDH|WHEN LOT CHANGES|2 LEVEL QC at 8 AM ,3 PM , 10 PM|3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax+b**|RIQAS_monthly|
|LIP|Fixed Factor(-500)|2 level pooled sera at 8 am|NA|**Literature factor=500**| |
|IRON|Calibration when lot changes|2 Level QC with each batch|3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax+b**|RIQAS_monthly|
|UIBC|Calibration when lot change and along with each batch. each batch calibration is minicalibration.|1 Level QC with each batch|3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax+b**|RIQAS_monthly|
|ADA|in every batch, with 60 U/ml ADA standard X 3 times replicate |Two level pooled sera in every batch|NA| |QA Forum|
|MPR|When Lot changes|two level diluted(1:100 times) calibrator sera at 8 am (In duplicate and second value is considered)|10 Calibrators cups X 2 time replicate and 5 blank cups X 2 time replicate|**y=ax**|CSF_RIQAS_monthly|
|CHOH|Fixed factor(caculated from CHO factor),change it when factor of cholesterol changes|2 LEVEL QC daily|NA|**(cholesterol factor)x1.1**|RIQAS_monthly|
|CHE| whenever new 30ml bottle is reconstituted| 2 LEVEL QC AT 8 AM, 1 LEVEL QC AT 3 PM, 1 LEVEL QC AT 10 PM| 4 calibrators cups X 2 time replicate and 4 QC cups X 2 time replicate and 2 blank cups X 2 time replicate |**y=ax**|RIQAS_monthly|
|PHO|When Lot changes|2 LEVEL QC at 8 AM ,3 PM , 10 PM(In duplicate and second value is considered)|3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax**|RIQAS_monthly|
|Dithionite Test|Qualitative test |Two level control (positive and negative) run in each batch|NA| |QA Forum_monthly| 
|Hemoglobin electrophoresis-Detection of HbA, HbS & HbF|Qualitative test |Control of HbA, HbS and HbF are run with each batch|NA| |QA Forum_monthly|
|Fluid Glucose|same as serum glucose |Same as serum Glucose IQC|3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax**|QA FORUM 6 monthly and in CSF RIQAS monthly |
|Fluid Protein (Biuret)|Same as serum protein|Same as Serum protein IQC |3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax**|QA Forum_6 monthly|
|Fluid Protein (Pyrogallol Red)|Same as MPR |Two level pooled fluid in every batch|10 serum Calibrators cups X 2 time replicate and 5 blank cups X 2 time replicate|**y=ax**|QA FORUM_6 monthly|
|Serum Ketone bodies| Qualitative Test|Two level pooled sera( Normal and Acetone spiked) Twice a day|NA| |QA Forum_6 monthly|
|Urine Ketone bodies| Qualitative Test| Same as serum ketone |NA| |QA Forum_6 monthly|
|Urine Calcium|Same as serum calcium calibration |1 level pooled spiked Acidify urine once when test done |3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax**|QA Forum_6 monthly|
|Urine Creatinine|Same as serum creatinine |1 level pooled spiked urine once when test done|3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax**|QA Forum_6 monthly|
|Urine Phosphorous|Same as serum Phosphorus |1 level pooled spiked Acidify urine once when test done|3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax**|QA Forum_6 monthly|
|Urine Sodium|When Lot changes |1 level pooled spiked urine once when test done|3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax**|QA Forum_6 monthly|
|Urine Protein (Biuret)|When Lot changes |1 level pooled spiked urine once when test done|3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax**|QA Forum_6 monthly|
|Urine Protein (Pyrogallol Red)|When Lot changes |2 level diluted calibrator once a day|10 serum Calibrators cups X 2 time replicate and 5 blank cups X 2 time replicate|**y=ax**|QA Forum_6 monthly|
|Urine Uric Acid (Uricase POD)|same  as serum Uric Acid |1 level pooled spiked basic urine once when test done|3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate|**y=ax**|QA Forum_monthly|
|Protein electrophoresis-Detection of M band|Qualitative Test |Three level QC run with each batch|NA| |QA Forum_6 monthly|
|CK(IFCC at 37)|When lot changes|2 LEVEL QC AT 8 AM, 1 LEVEL QC AT 3 PM, 1 LEVEL QC AT 10 PM|1 kit standard cup and 1 blank cup 5times replicated|**y=ax+b**|RIQAS monthly|
|CRP(turbidimetry)|When lot changed|2 level in-house pool in morning 8 am|serial dilution of kit standard      {{ :nchsls:c:biochemistry:document:how_to_do_serial_dilution_of_crp_standard.ods |}} 5 cups with 4 replicates|**y=ax+b**|QA Forum 6 monthly|
|DDIMER(turbidimetry)|When lot changed|1 level diluted kit standard in morning 8 am| serial dilution of kit standard 5 cups with 1replicate{{:nchsls:c:biochemistry:document:how_to_do_serial_dilution_of_ddimer_standard.ods |}}|**y=ax**|QA Forum 6 monthly|
|Total 25-OH Vit-D(ECLIA)|When lot changed|IQC weekly one level|Two level calibrator freeze dried form reconstitute with 1ml qc water|Autocalibrated|RIQAS monthly|
|Ferritin| When lot changed or Calibration expired|IQC weekly one level|Three level calibrator freeze dried form reconstitute with 1ml qc water|Autocalibrated|RIQAS monthly|
|Procalcitonin| When lot changed or Calibration expired|IQC weekly one level|Two level calibrator ready to use(Frozen sample)|Autocalibrated|QA Forum 6 monthly|
|TSH| When lot changed or Calibration expired|2 level IQC daily at 8AM, 1 level at 3 PM, 1 level at 10 PM|Three level calibrator ready to use|Autocalibrated|RIQAS monthly|
|FT3| When lot changed or Calibration expired|IQC weekly one level|Three level calibrator freeze dried form reconstitute with 1ml qc water|Autocalibrated|RIQAS monthly|
|FT4| When lot changed or Calibration expired|IQC weekly one level|Three level calibrator freeze dried form reconstitute with 1ml qc water|Autocalibrated|RIQAS monthly|
|LH| When lot changed or Calibration expired|IQC weekly one level|Two level calibrator ready to use(Frozen sample)|Autocalibrated|RIQAS monthly|
|FSH| When lot changed or Calibration expired|IQC weekly one level|Two level calibrator ready to use(Frozen sample)|Autocalibrated|RIQAS monthly|
|Prolactin| When lot changed or Calibration expired|IQC weekly one level|Two level calibrator freeze dried form reconstitute with 1ml qc water|Autocalibrated|RIQAS monthly|
|NTProBNP2| When lot changed or Calibration expired|IQC weekly one level|Three level calibrator ready to use|Autocalibrated|Not done|
|HsTnI| When lot changed or Calibration expired|IQC weekly one level|Three level calibrator ready to use|Autocalibrated|QA Forum 6 monthly|
|B-HCG| When lot changed or Calibration expired|IQC weekly one level|Three level calibrator freeze dried form reconstitute with 1ml qc water|Autocalibrated|RIQAS monthly|
|Vit B12| When lot changed or Calibration expired|Not done because no sample received |Two level calibrator ready to use(Frozen sample)|Autocalibrated|RIQAS monthly|
|CoV2G| When lot changed or Calibration expired|Not done|Single level calibrator ready to use|Autocalibrated|Not done|
|Lactate| One point autocalibration half hourly, two point autocalibration at 2 hourly |one level IQC daily|One point autocalibration half hourly, two point autocalibration at 2 hourly|-|Not done|
|HCO3| One point autocalibration half hourly, two point autocalibration at 2 hourly |one level IQC daily|One point autocalibration half hourly, two point autocalibration at 2 hourly|-|Not done|
|pH| One point autocalibration half hourly, two point autocalibration at 2 hourly |one level IQC daily|One point autocalibration half hourly, two point autocalibration at 2 hourly|-|Not done|
|pCO2| One point autocalibration half hourly, two point autocalibration at 2 hourly |one level IQC daily|One point autocalibration half hourly, two point autocalibration at 2 hourly|-|Not done|
|pO2| One point autocalibration half hourly, two point autocalibration at 2 hourly |one level IQC daily|One point autocalibration half hourly, two point autocalibration at 2 hourly|-|Not done|
|HbA1C|Weekly and When buffer changed|2 level IQC from in house pool with each batch.|Two level calibrator freeze dried form reconstitute with 4ml qc water|-|QA Forum 6 monthly|



^Erba Chem-5 Plus V2^
^Parameter^Calibration^ ^IQC^ ^
^ ^When in use(Erba XL-640 breakdown)^When not in use^When in use(Erba XL-640 breakdown)^When not in use^
|GLC|Every batch|Every month before External QC and when lot changes|2 level QC With Every batch|2 level QC every Sunday|**y=ax**|
|BIL|when lot changes|Every month before External QC and when lot changes|2 level QC 3 times a day|2 level QC every sunday|**y=ax**|
|CR|when lot changes|Every month before External QC and when lot changes|2 level QC 3 times a day|2 level QC every sunday|**y=ax**|
|ALT|when lot changes|Every month before External QC and when lot changes|2 level QC 3 times a day|2 level QC every sunday|**y=ax**|
|ADA|in every batch|Not applicable|Two level pooled sera in every batch|Not applicable|